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- SEROLOGICAL TEST METHOD
Though it has been used to be the 'gold' standard, it is now being superseded by molecular biological methods as they become more precise and time efficient.
- MOLECULAR BIOLOGICAL TEST METHOD
Under rapid development and widely accepted in various laboratories.
- Complement-mediated Microlymphocytotoxicity
- Easy to test without necessity of expensive equipments.
- High-quality serum required to obtain low resolution results and reliable results
- Large volume of blood & viable lymphocytes is required.
- Difficult to find high-quality serum for detecting rare antigens found in different races.
- PCR (Polymerase Chain Reaction) has typically been used
- All of the molecular methods require good quality genomic DNA
- Requires extra primers to be used
- Sequence of alleles must be known
- SSO: sequence specific oligonucleotide
- reverse SSO hybridization
- RFLP: restriction fragment length polymorphism
- SSCP: sequence specific conformational polymorphism
- SSP: sequence specific primer (currently applied)
- SBT: sequence-based typing (currently applied)
- Recently, SPREX(Sequence specific Primer Refractory Extension) chips and real-time PCR methods have independently been developed and used for HLA test.
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